Food Standards (Application A1318 – Steviol glycosides produced by enzymatic conversion using enzymes produced by GM Escherichia coli BL21) Variation

The Board of Food Standards Australia New Zealand gives notice of the making of this variation under section 92 of the Food Standards Australia New Zealand Act 1991. The variation commences on the date specified in clause 3 of this variation.

Dated 13 October 2025.

MATTHEW O’MULLANE, Delegate of the Board of Food Standards Australia New Zealand.

1 Name

This instrument is the Food Standards (Application A1318 – Steviol glycosides produced by enzymatic conversion using enzymes produced by GM Escherichia coli BL21) Variation.

2 Variation to Standards in the Australia New Zealand Food Standards Code

The Schedule varies Standards in the Australia New Zealand Food Standards Code.

3 Commencement

The variation commences on the date of gazettal.

Schedule

Schedule 3—Identity and purity

[1] Subparagraph S3—35(2)(f)(ii)

Omit the subparagraph, substitute:

(ii) sucrose synthase (EC 2.4.1.13) sourced from Escherichia coli K-12;

(g) by enzymatic conversion of purified stevia leaf extract to produce rebaudioside M using a combination of enzymes that contains:

(i) UDP-glucosyltransferases from Stevia rebaudiana sourced from Escherichia coli BL21; and

(ii) sucrose synthase (EC 2.4.1.13) from Arabidopsis thaliana sourced from Escherichia coli BL21.

Schedule 18—Processing aids

[2] Subsection S18—9(3) (table)

Insert the following entry for each enzyme in alphabetical order: